Figure 4.

Enhancing the immunogenicity of a protein carrier through disulfide oxetane stapling. a) The functional stapling of CRM₁₉₇ (pdb ID CRM₁₉₇: 4AE0) with 1. b) SDS‐page of native and stapled CRM₁₉₇ ‐1. c) Far UV CD spectrum. d) Near UV CD spectrum. e) DCS analysis. f) Competition of anti‐CRM₁₉₇ serum binding to the protein with CRM₁₉₇ and its stapled form as inhibitors. g) Anti‐CRM₁₉₇ IgG levels of CRM₁₉₇ and CRM₁₉₇‐1 after first and second boost immunizations in mice, 2 weeks apart.