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. 2017 Nov 17;8:2196. doi: 10.3389/fmicb.2017.02196

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Copyright © 2017 Cao, Realegeno, Pant, Satheshkumar and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Resveratrol suppresses N1L-deleted VACV replication. (A) Deletion of N1L gene from recombinant virus was confirmed by PCR with two pairs of primer specific for C11R gene (positive control) and N1L gene, respectively. (B) HeLa cells were infected with wild type and N1L-deleted VACV at an MOI of 1. Virus growth was determined by a plaque assay. (C) HeLa cells were infected with N1L-deleted VACV at an MOI of 1 in the presence or absence of resveratrol (50 μM). Virus titers were determined by a plaque assay at 24 hpi. The asterisk indicates significant difference (P < 0.05). The error bar indicates standard deviation.