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Detect the changes in Treg in spleen by FACS. Tregs were stained by CD4 and Foxp3 antibodies. After mononuclear cells being gated, the double positive cells in outer and upper quadrant were defined as Tregs. TBI significantly decreased the level of Tregs at 3 and 7 days after injury, but EPO treatment significantly increased Tregs level at 3 and 7 days after injury. n = 6/group, *p < .05 TBI + EPO group versus TBI + Saline group, #p < .05 versus Sham group
