Figure 3.

LC-MS/MS analysis of methoxyestrone metabolites (2-MeOE₁, 4-MeOE₁) in MCF-7 cells after treatment with isoflavones. MCF-7 cells were treated for 48 h with the isoflavones, GN, DZ, BA, and FN, followed by 24 h treatment with E₂ (1 μM). 2-MeOE₁ and 4-MeOE₁ metabolites were analyzed by LC-MS/MS. (A, B) Effect of the ER agonists, GN and DZ, on (A) 2-MeOE₁ and (B) 4-MeOE₁ metabolites. (C, D) Effect of the AhR agonists, BA and FN, on (C) 2-MeOE₁ and (D) 4-MeOE₁ metabolites.