Skip to main content
. Author manuscript; available in PMC: 2017 Nov 22.
Published in final edited form as: Nanomedicine. 2016 Aug 20;13(2):411–420. doi: 10.1016/j.nano.2016.08.006

Figure 1. Design and purification of F epitope-fused N-nanorings (N-FsII).

Figure 1

F antigenic site II (FsII) was fused to the N-terminus (Nter) or at the top of a β-hairpin between G106 and K107 residues (G106) of N. (A) Schematic representation of the two N-FsII constructs, based on the 3D structure of N-nanorings. FsII is highlighted in red on the structure of a postfusion RSV F monomer (adapted from Swanson et al)10 and is represented by a red sphere on N-nanorings. N-FsII proteins were analyzed by SDS-PAGE (B) or Native Gel Electrophoresis (NGE) (C), in comparison with FsII-free N-nanorings (N). (D) The hydrodynamic radius of the two purified N-FsII was estimated by Dynamic Light Scattering (DLS). DLS plot is representative of 2 independent measures. (E) Western blot assay using anti-N polyclonal antibodies or FsII-specific monoclonal antibodies (mAb11 and AbD Serotec). The relative molecular mass (kDa) is indicated on the left.