REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-GFP	Thermo Fisher Scientific	Cat# A-11122; RRID: AB_221569
Mouse monoclonal anti-bruchpilot	A. Hofbauer, University of Regensburg; Bavaria; Germany	Cat# nc82; RRID: AB_2314865
Rat monoclonal anti-RFP	ChromoTek	Cat# 5f8-100, RRID: AB_2336064
FITC-AffiniPure Donkey polyclonal anti-Rabbit IgG (H+L)	Jackson ImmunoResearch Labs	Cat# 711-095-152, RRID: AB_2315776
Cy3-AffiniPure Donkey Anti-Rat IgG (H+L)	Jackson ImmunoResearch Labs	Cat# 712-165-153, RRID: AB_2340667
Cy5-AffiniPure Donkey Anti-Mouse IgG (H+L)	Jackson ImmunoResearch Labs	Cat# 715-175-151, RRID: AB_2340820
Experimental Models: Organisms/Strains
D. melanogaster: w1118	Bloomington	RRID: BDSC_5905
D. melanogaster: Df(2R)BSC305	Bloomington	RRID: BDSC_23688
D. melanogaster: Df(2R)BSC700, P+PBac{w[+mC] = XP3.RB5}BSC700	Bloomington	RRID: BDSC_26552
D. melanogaster: Dh44-R1 RNAi TRiP.JF03208 (attP2)	Bloomington	RRID: BDSC_28780
D. melanogaster: Dh44-R2 RNAi TRiP.JF03289 (attP2)	Bloomington	RRID: BDSC_29610
D. melanogaster: elav[C155]; UAS-Dicer2	Bloomington	RRID: BDSC_25750
D. melanogaster: UAS-Dicer2	Bloomington	RRID: BDSC_24650
D. melanogaster: UAS-Dicer2	Bloomington	RRID: BDSC_24651
D. melanogaster: Dh44-R1 R21A07-GAL4 (attP2)	Bloomington	RRID: BDSC_49855
D. melanogaster: hugin-GAL4	Bloomington	RRID: BDSC_58769
D. melanogaster: hugin-LexA (GMR61H10-lexA (attP40))	Bloomington	RRID: BDSC_52715
D. melanogaster: hugin RNAi TRiP.JF03122 (attP2)	Bloomington	RRID: BDSC_28705
D. melanogaster: UAS-reaper	Bloomington	RRID: BDSC_5823
D. melanogaster: UAS-nls.GFP	Bloomington	RRID: BDSC_7032
D. melanogaster: UAS-Denmark, UAS-syt.eGFP	Bloomington	RRID: BDSC_33064
D. melanogaster: UAS-Denmark	Bloomington	RRID: BDSC_33062
D. melanogaster: 13XLexAop2-GFP-Rab3 (VK00027)	Bloomington	RRID: BDSC_52239
D. melanogaster: 13XLexAop2-IVS-GCaMP6m-p10 (attp1)	Bloomington	RRID: BDSC_44275
D. melanogaster: vglut-GAL4 (ok371)	Bloomington	RRID: BDSC_26160
D. melanogaster: Dh44-GAL4 (attp2)	Vienna Drosophila Resource Center	VDRC: 207474 Flybase: FBti0169412
D. melanogaster: Dh44-R1 RNAi kk	Vienna Drosophila Resource Center	VDRC: 110708 Flybase: FBtp0067029
D. melanogaster: Hugin RNAi GD	Vienna Drosophila Resource Center	VDRC: 26766 Flybase: FBtp0025825
D. melanogaster: Dh44-R2 RNAi NIG	NIG-Fly (National Institute of Genetics Mishima, Japan)	NIG: 12370R-4 Flybase: FBtp0074111
D. melanogaster: per01	[61]	Flybase: FBal0013649
D. melanogaster: pdf01	[3]	Flybase: FBal0102946
D. melanogaster: pdfrhan5304	[14]	Flybase: FBal0218040
D. melanogaster: Dh44-R1-GAL4	[18]	Flybase: FBtp0112068
D. melanogaster: neurexin-GRASP (LexAop-CD4-spGFP11; UAS-nrx-spGFP1-10)	[28]	N/A
D. melanogaster: CD4-GRASP (LexAop-CD4-spGFP11; UAS-CD4-spGFP1-10)	[62]	N/A
D. melanogaster: UAS-P2X2	[30]	Flybase: FBtp0021869
REAGENT or RESOURCE	SOURCE	IDENTIFIER
D. melanogaster: UAS-Kir2.1	[32]	Flybase: FBtp0014166
D. melanogaster: UAS-TrpA1	[19]	Flybase: FBtp0040248
D. melanogaster: UAS-ANF-GFP	[41]	Flybase: FBtp0016739
D. melanogaster: vasa-Cas9	Bloomington	RRID: BDSC_51323
D. melanogaster: Dh44-R1DsRed	This paper	N/A
D. melanogaster: Dh44-R2174	This paper	N/A
D. melanogaster: Dh44-LexA (attp40)	This paper	N/A
D. melanogaster: UAS-t-Dh44 (attp40)	This paper	N/A
Recombinant DNA
Plasmid: pBPLexA::p65Uw	[63]	Addgene plasmid# 26231
Plasmid: pCFD4-U6:1_U6:3tandemgRNAs	[64]	Addgene plasmid# 49411
Plasmid: pDsRed-attP	Melissa Harrison & Kate O’Connor-Giles & Jill Wildonger	Addgene plasmid# 51019
Plasmid: pJFRC7-20XUAS-IVS	[63]	N/A
Software and Algorithms
Fiji	[65]	https://fiji.sc/
ClockLab	Actimetrics	http://actimetrics.com/products/clocklab/
Graphpad Prism (v 7.03)	Graphpad Software	https://www.graphpad.com/