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. 2017 Nov 21;22:48. doi: 10.1186/s40001-017-0291-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Detection of tumor cell apoptosis with TUNEL staining (original magnification ×400). The number of apoptotic tumor cells in each of the four groups varied (a saline; b 5-FU-loaded nanobubbles; c 5-FU-loaded nanobubbles with non-low-frequency ultrasound; d 5-FU-loaded nanobubbles with low-frequency ultrasound)