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. 2017 Nov 21;17:779. doi: 10.1186/s12885-017-3750-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Surface marker expression in Rb Y79 cell line using flow cytometry. Gating of single cells and voltage channel setting- (a) FSC vs SSC plot, (b) and (c) Doublet discrimination plots (d) Unstained control. Analysis of various surface markers with representative distribution of various sub-populations within the cell line (e) CD133-APC (f) CD44-PE (g) CD90-FITC, (h) CXCR4-APC (i) ABCB1-FITC. j and (k) Purity of sorted CD133^lo and CD133^hi cells was recorded as >90%. Cell cycle analysis of the total Y79 cells, CD133^lo and CD133^hi subsets highlighting the G0/G1 status of CD133^lo cells (l) Cell cycle distribution of total Y79 cells (m) CD133^lo cells predominantly observed in the G0/G1 phase (83.3 ± 4.1%) (n) CD133^hi cells mainly observed in the proliferating S/G2/M phase (81.1 ± 4.1%)