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. 2017 Oct 30;114(46):E9913–E9922. doi: 10.1073/pnas.1707779114

Fig. 1.

Fig. 1.

Production of hPGCLCs from primed pluripotency hiPSCs via 72-h reprogramming toward naïve pluripotency. (A) Diagram of the 11-d schedule of hPGCLC production from primed pluripotency hiPSCs. (B) Phase contrast images of hiPSCs before and after reprograming toward naïve pluripotency in the 4i medium and day 5 EBs. (C) mRNA expression of TET1 and DNA methyltransferase genes in the primed and 4i reprogrammed hiPSCs. TaqMan real-time qPCR measurements (n = 3, mean ± SD). (D) FACS enrichment of CD38+ and CD38− EB cells from days 5 and 8 EBs. FSC, forward scatter. (E) mRNA expression of germline markers in EBs. TaqMan real-time qPCR measurements (n = 6, mean ± SD).