Fig. 2.

Transcriptomal profiles of CD38⁺ hPGCLCs and precursors. (A and B) Unsupervised hierarchical clustering of CD38⁺ hPGCLCs and their precursors based on transcriptomes (A) or 24 marker genes (B). Details of the three gene clusters 1–3 indicated in A are shown in Fig. S1 C1–C3, respectively. RNA-seq data were obtained from three independently performed experiments. Gray color indicates “zero value” data, reflecting the absence of expression. (C) Transcriptomal PCA. Arrows indicate the path of hiPSC differentiation to CD38⁺ hPGCLCs and CD38⁻ EB cells. (D) RNA-seq tracks at the 3′-UTR of PRDM1 mRNA. Positions of exons 6–8, 3′-UTR, and miRNA binding sites are indicated. (E) GO analysis of DEGs between CD38⁺ hPGCLCs and CD38⁻ EB cells.