Fig. 6.

Effects of CXCL12 on mRNA expression in hPGCLCs. (A) Expression of mRNA transcripts in CD38⁺ hPGCLCs or CD38⁻ EB cells at day 8 EBs. EBs were cultured in the standard condition until day 5 and then incubated for an additional 3 d in the presence or absence of Rho Kinase Inhibitor (ROCKi) and/or CXCL12 in the PGCLC production medium. Amounts of mRNA expression are shown as counts per million (cpm) values of normalized reads of three repeated RNA-seq experiments. (B and C) GO analysis of DEGs in hPGCLCs produced in the ROCKi-deficient medium in the presence or absence of CXCL12. Three RNA-seq experiments determined 1,190 up-regulated (B) and 735 down-regulated (C) DEGs. Statistically significant GO terms (FDR < 5%) are shown. (D) RNA-seq traces of CXCR4, PTGER3, BCL2, and CASP8 mRNA expression. The height of peaks represents relative mRNA expression of a gene in the six cell culture conditions and was normalized for each gene. ZRAMB2 locates adjacent to PTGER3 and is shown as control. KDSR and VPS4B are controls located adjacent to BCL2. ACTB expression shows quantitative reproducible RNA-seq experiments across the six different cell culture conditions. Note that PTGER3 and BCL2 traces are shown only at representative exons.