Fig. 1.

COP1 interacts with WDL3 in the dark. (A) Interactions between WDL3 and COP1, or COP1 domain-deletion mutant forms in the yeast two-hybrid system. (B) In vitro pull-down assays showing that GST-WDL3 interacts with MBP-COP1. MBP-COP1 pulled down by GST-WDL3 was detected by anti-MBP antibody. GST and GST-MAP65-1 were used as controls and did not interact with MBP-COP1. (C) Interaction of COP1 with WDL3 was revealed via firefly LCI assay in tobacco leaves in the dark. Negative controls used were nLuc+cLuc-COP1, WDL3-nLuc+cLuc, and nLuc+cLuc. (Scale bar: 1 cm.) (D) Coimmunoprecipitation of COP1 and WDL3. Total protein was extracted from Arabidopsis protoplasts expressing 35S:COP1-MYC and 35S:WDL3-GFP. Anti-MYC-Agarose Affinity Gel was used for immunoprecipitation, and the precipitated proteins were analyzed by immunoblots using an anti-GFP antibody.