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COP1 directly ubiquitinates WDL3 in vitro. In vitro ubiquitination assays showing that WDL3 is ubiquitinated by COP1. Purified MBP, recombinant MBP-COP1, denatured COP1 (bMBP-COP1), and GST-WDL3 were used in the assays. The ubiquitination of WDL3 and WDL3 1-195 was analyzed by immunoblots using anti-GST and anti-FLAG antibodies. GST-HYH was used as a positive control, and GST-WDL5 was used as a negative control.
