Skip to main content
. 2017 Oct 30;114(46):12231–12236. doi: 10.1073/pnas.1713225114

Fig. 3.

Fig. 3.

Relationship between DSB hotspot location and chromatin state. (A) Nucleosome occupancy at the sites of DSB hotspots measured using micrococcal nuclease digestion. (B) Overlap of DSB hotspots with H3K4me3 sites. (C) Immunocolocalization of H3K4me3 marks and RAD51 foci in a maize meiocyte at zygotene. Image is a flat projection of five consecutive optical sections of a 3D nucleus. (D) Comparison of cytosine methylation levels (CG, CHG, and CHH combined) detected using a ChIP-microarray analysis in a 2-kb window around DSB hotspots, CO sites, and random genome sites. (E) Immunocolocalization of H3K9me2 and RAD51 in a maize meiocyte at zygotene showing limited overlap of DSBs with H3K9me2 sites. Image is a flat projection of five consecutive optical sections through a 3D nucleus.