Fig. 2.
Representative tissue distribution and replication of ZIKV isolates in organotypic mouse brain slices. (A) Brain slice cultures from E15 embryos were infected with 105 pfu of the ZIKV PRV isolate or uninfected and at 3 dpi, were fixed and stained with pan-flavivirus antibody against the E glycoprotein (ZIKV-E) and DAPI. ZIKV-E signal demonstrated ZIKV infection in focal areas, including the developing neocortex and midbrain. (Scale bar: 500 μm.) (B) Higher magnification (60× objective) of the ventricular surface of infected brain slice culture, stained with pan-flavivirus ZIKV-E antibody. The location of these cells along the ventricle in the ventricular zone, and their morphology with apical and basal processes all support that these ZIKV-E positive cells are radial glia. (Scale bar: 10 μm.) (C) Time course of replication of multiple isolates of ZIKV in E15 brain slice cultures. Results are representative of three independent experiments.