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Increasing permissiveness of transformed focus formation by serial selection of their cellular microenvironment (15). Two lineages, A and B, underwent a 1° assay in 10% CS for 3 wk, followed by 2° and 3° assays in 2% CS for 3 wk. The 3° assay at 105 cells is shown, as is that of 104 cells combined with 105 cells that had not undergone a selective assay to serve as a background for focus formation. Reproduced from ref. 15.
