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. 2017 Oct 30;114(46):E9903–E9912. doi: 10.1073/pnas.1716009114

Fig. 2.

Fig. 2.

FiVe1 irreversibly inhibits stemness-associated properties in mesenchymally transformed breast cancer cells. (A) Quantification of wound closure in an in vitro scratch assay with FOXC2-HMLER cells (n = 3, mean and SD, FiVe1, 1 μM). (B) Growth curves of FOXC2-HMLER cells treated with 1 μM FiVe1 during days 1–4 as noted by dashed vertical lines (n = 3, mean and SD). (C) Quantification of mammospheres from an in vitro mammosphere formation assay with the indicated cell types. Pretreatment indicates a 72-h treatment with 1 μM FiVe1 before plating in mammosphere formation conditions. During assay indicates 1 μM compound treatment during the mammosphere assay (14 d) (n = 3, mean and SD). (D) Representative images of FOXC2-HMLER cells treated for 24 h with DMSO or 500 nM FiVe1 and then stained with DAPI and anti-VIM antibodies. (Scale bar, 10 μm.) (E) Quantification of multinucleation (n > 3 nuclei per cell) in the indicated cell types treated with 500 nM FiVe1 for 24 h (n = 3, mean and SD; *P < 0.05, **P < 0.005, ***P < 0.0005; t test).