Table 3.

miRNAs validation by qRT-PCR compared with sequencing data.

miRNA species	Samples	Absolute expression level by qRT-PCR(μM)	RPM detected by sequencing
miR-322-3p	LIR	3.89E-08±1.70837E-09	15.472
	DM	1.43E-08±6.42968E-10	1.7822
miR-194-5p	DM	4.72798E-08±5.92621E-09	9.8505
	OVX	3.27459E-07±3.87998E-09	1.5392
miR-9a-5p	LIR	9.83764E-08±8.69045E-09	71.5827
	OVX	4.54503E-08±1.68551E-09	10.3122

qRT-PCR – quantitative polymerase chain reaction; RPM – reads per million; OVX – ovariectomized; DM – diabetes mellitus; LIR – liraglutide.