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. 2017 Nov 17;8:829. doi: 10.3389/fphar.2017.00829

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Copyright © 2017 Su, Li, Yu, Yang, Deng, Su, Zeng, Chen, Zhang and Lai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemistry detection for related proteins of Th cells. (A) Representative IHC picture (400× magnification) for CD4 (Th), IFN-γ (Th1), IL-17A (Th17), and Foxp3 (Treg), the positive area was indicated by red arrows. (B–E) The mean ratios of integrated optical density (IOD) to area (IOD/pixel) were used for analyzing the results of immunohistochemistry. Values were represented the means ± SD (n = 6). ^#p < 0.05 and ^##p < 0.01 versus Normal group, ^∗p < 0.05 and ^∗∗p < 0.01 versus Model group.