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. Author manuscript; available in PMC: 2018 Jan 11.
Published in final edited form as: Cell Host Microbe. 2016 Dec 22;21(1):59–72. doi: 10.1016/j.chom.2016.11.002

Figure 7. SMURF1 Controls Mycobacterial Growth in Human Macrophages, is Expressed in Granulomas, and Colocalizes with M. tuberculosis in Lungs of Patients with Active Pulmonary Tuberculosis.

Figure 7

(A) Smurf1 knockdown in primary human monocyte-derived macrophages transduced with controls or two different SMURF1 shRNA constructs. mRNA levels of SMURF1 were analyzed by quantitative RT-PCR. Bars represent mean ± SEM (normalized to control scrambled shRNA). Similar results were observed in three independent experiments. ****P<0.0001; t-test.

(B) Mtb growth in primary human monocyte-derived macrophages transduced with control scrambled or SMURF1shRNA. Infected cells were lysed at the indicated time-points and Mtb growth was determined by counting CFUs. Bars are mean ± SEM of quadruplicate samples; each sample was normalized to day 0. Similar results were observed in two independent experiments. See Figure S5A for results from one additional donor. *P<0.05; **P<0.01 for indicated comparison; two-way ANOVA.

(C) Immunohistochemical staining for SMURF1 in lung biopsies of two human patients with active pulmonary tuberculosis or from a normal lung. Scale bars, 100 μm.

(D) Immunofluorescence staining of a lung biopsy of a human patient with active pulmonary tuberculosis using anti-Mtb and anti-SMURF1 antibodies. Left panel, image of Mtb that colocalizes with SMURF1. See Figure S5 for additional examples. Right panel, image of Mtb that do not colocalize with SMURF1. Scale bar, 5 μm. See also Figure S5.