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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: DNA Repair (Amst). 2017 Oct 23;60:64–76. doi: 10.1016/j.dnarep.2017.10.008

Figure 5. Electrophoretic mobility shift assays for the binding of RAD51 variant and wild-type proteins to ϕX174 ssDNA and dsDNA in the presence of 1 mM ATP.

Figure 5

(A–B) Concentrations of RAD51 WT or E258A as indicated were incubated with 20 μM ϕX174 circular ssDNA (CSS) or linear dsDNA (LDS) then electrophoresed as described in Materials and Methods. (C–D) Binding reactions identical to Panels A–B except that RAD51 F86L replaced E258A.