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Apparent dissociation constants for RAD51-DNA interactionsa.
| Kd (μM) | WT | F86L | E258A |
|---|---|---|---|
| ssDNA | 1.9 ± 0.2 | N.D.b | N.D.b |
| ssDNA + ATP | 0.6 ± 0.1 | 4.9 ± 0.4 | 3.1 ± 0.3 |
| dsDNA | 0.8 ± 0.1 | 1.7 ± 0.1 | 2.1 ± 0.1 |
| dsDNA + ATP | 0.7 ± 0.1 | 1.8 ± 0.2 | 1.3 ± 0.1 |
Apparent Kd values were estimated from curve fits of data in Figure 6, as described in Materials and Methods.
Not Determined-- Measuring Kd required unattainably high protein concentrations.
