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Perls’ Prussian blue iron staining of MSCs labeled with Ferumoxytol under different conditions: (A) using the traditional method (Protocol 1); (B) co-incubation with non-adherent cells and supernatant liquid (Protocol 2); (C) co-incubation with non-adherent cells; and (D) co-incubation with supernatant liquid. Blue arrows point to Prussian blue positive cells. Red arrows show Prussian blue negative cells. [Modified from Figures 3, 4, and 6 of (Liu et al., 2016)].
