Fig. 1.

Evaluating the reliability and consistency of different reporter systems in Y. lipolytica. (A) hrGFP fluorescence protein as the reporter. MFI, mean fluorescence intensity. Four biological replicates were tested. (B) mCherry fluorescence protein as the reporter. Four biological replicates were tested. (C) β-glucuronidase (GUS) reporter with X-gluc (5-Bromo-4-chloro-3-indolyl-β-D-glucuronide) as substrate. Blue product could be quantified based on a spectrophotometric absorbance assay. (D) β-glucuronidase (GUS) reporter with MUG (4-methylumbelliferyl-beta-D-glucuronide) as substrate. Fluorescence product could be quantified with a fluorometric assay. Four biological replicates were tested. (E) Luciferase Nanoluc as the reporter. MLI, mean luminescence intensity. Three biological replicates were tested.