Fig. 3.
Shake-flask evaluation of the effect of crc deletion on muconate production from p-coumarate by engineered P. putida KT2440 strains in the presence of glucose. Cultures were grown in M9 minimal medium containing p-coumarate and fed glucose periodically as a source of carbon and energy for growth and sampled to evaluate culture growth by OD600 and the concentration of metabolites in the medium by HPLC. Each value represents the average of three biological replicates. The error bars represent standard deviation of the measurements. (A) KT2440-CJ102, (B) KT2440-CJ238, the Δcrc derivative of KT2440-CJ102.