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. 2016 Nov 7;14(9):1241–1251. doi: 10.1080/15476286.2016.1236170

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© 2017 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 2. — LC-MS analysis confirms that Dnmt2 methylates a tRNA containing a deoxycytidine at position 38. (A) Fragmentation patterns and mass transitions used for scanning of rm⁵C and dm⁵C in LC-MS. (B) LC-MS analysis of all-ribo tRNA^Asp and hybrid tRNA^AspdC38 before and after in vitro methylation by human Dnmt2.