Table 1.
Components of the m6A methyltransferase complex and their biologic roles.
| METTL3 | ||
|---|---|---|
| Human | METTL3 | •METTL3 KD leads to circadian clock period elongation.81 |
| •METTL3 promotes translation independently of its catalytic activity.34 | ||
| •METTL3 KD prevents differentiation of hESC.74 | ||
| Mouse | Mettl3 | •Mettl3 KO in naïve mESC leads to hyper naïve ground state, while in primed mESC boost cell differentiation.32 |
| •Mettl3 KO leads to embryonic lethality.32 | ||
| Zebrafish | METTL3 | •Morpholino depletion leads to developmental defects during embryogenesis.8 |
| A. thaliana | MTA | •MTA disruption results in embryonic lethality.30 |
| •MTA reduction leads to various developmental and organ definition defects.31 | ||
| S. cerevisiae | Ime4 | •Ime4 is required for sporulation and meiosis.64,82 |
| D. melanogaster | Ime4 | •Ime4 inactivation leads to defects during oogenesis.83 |
| •Ime4 KO affects fly locomotion due to impaired neuronal function.21,22 | ||
| •Ime4 regulates splicing of Sxl and fine tunes sex determination.21,22 | ||
| METTL14 | ||
| Human | METTL14 | •Structural component of the methyltransferase complex.7,8,11-13 |
| S. cerevisiae | Kar4 | •Transcriptional activator required for karyogamy.35,84 |
| D. melanogaster | Mettl14 | •Mettl14 KO affects fly locomotion due to impaired neuronal functions.22 |
| •Mettl14 regulates splicing of Sxl and fine tunes sex determination.21, 22 | ||
| WTAP | ||
| Human | WTAP | •Structural component of the mehyltransferase complex required for METTL3-METTL14 stabilization.7,8 |
| Mouse | WTAP | •WTAP KO results in early embryonic lethality.39 |
| Zebrafish | WTAP | •WTAP morpholinos display defects in head and brain development.8 |
| S. cerevisiae | Mum2 | •Mum2 is required for meiotic mRNA methylation as part of the MIS complex (Mum2, Ime4, Slz1).82 |
| D. melanogaster | Fl(2)d | •Fl(2)d is required for splicing of Sxl and its KO leads to embryonic lethality.41 |
| •Fl(2)d controls retinal development.44 | ||
| •Structural component of the methyltransferase complex, required for Ime4-Mettl14 stabilization.22 | ||
| RBM15, RBM15B | ||
| Human | RBM15,RBM15B | •RBM15 fusion with MKL1 is associated with acute megakaryoblastic leukemia.50 |
| •RBM15 and RBM15B are components of the methyltransferase complex, responsible for complex recruitment to targeted sites.15 | ||
| Mouse | RBM15, RBM15B | •Loss of RBM15 leads to embryonic lethality.47 |
| •RBM15 controls hematopoiesis, B-cell and megakaryocyte differentiation.47–49 | ||
| D. melanogaster | Nito | •Nito regulates wingless signaling and photoreceptor development.51,52 |
| •Nito is required for splicing of Sxl.53 | ||
| •Component of the methyltransferase complex.22 | ||