Fig 4. The maximum ROS production of Drosophila melanogaster harboring Alstonville (Alst) and Japan (Jap) mtDNA in the w¹¹¹⁸ genetic background.

Flies were aged 11 (upper chart) and 25 d (lower chart). The protein: carbohydrate (P:C) diets were 1:2, 1:4, 1:8 and 1:16. (A) Males. (B) Females. Maximum ROS production of female flies harboring the two mtDNA types did not differ, so they were pooled. Data for each mitotype is presented in S2 Fig. Bar represents basal ROS production, and error bars show the standard error of the mean.