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. 2017 Nov 22;6:e29425. doi: 10.7554/eLife.29425

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© 2017, Mead et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 5. — (a) Representative transmission electron microscopy images of zebra finch, Daubenton’s bat, Oyster toadfish and Diamondback rattlesnake SFMs (upper row) and intraspecific skeletal muscles (lower row). Blue arrows indicate SR, yellow arrows myofibrils. Rattlesnake muscle images adapted from Schaeffer et al., 1996. (b) Volumetric percentage estimates (VPE) of myofiber composition (see Materials and methods) show that all SFM have VPE_SR ≥15%, and SR per myofibril ratio ≥30%. Both VPE_SR and SR per myofibril ratio are significantly higher compared to fast intraspecific skeletal fibers for zebra finch, bat and rattlesnake (*, p<0.05). Data presented in descending order of SR per myofibril ratio. The numbers 1–8 above the bars refer to images in panel (a).