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Affinity isolation of 4a1 and HMBA bound proteins. (A) Procedure for the purification of 4a1 and HMBA bound proteins from LNCaP cell lysate. (B) The protein bands pullout by the probes. Visualization of the separated proteins with coomassie blue stain showed two major the proteins bound to 4a1 or HMBA. (C) Cell lysate exposed to a negative probe and the visualization of the separated proteins with coomassie blue stain showed no visible protein bands.
