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Necrosis induced by TPT and TPT + ascorbic acid in MCF-7 breast cancer cells at 48 h. MCF-7 cells were treated with TPT (0.5 μM) in the presence or absence of ascorbic acid (1 mM). Cells were collected and washed twice with ice-cold PBS and analyzed. Panel 1, control, no treatment; panel 2, ascorbic acid; panel 3, TPT; and panel 4, TPT + ascorbic acid. A representative scan is shown here.
