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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2017 Oct 7;113:244–254. doi: 10.1016/j.freeradbiomed.2017.10.008

Fig. 9.

Fig. 9

Measurement of DLDH activity (A) and protein content (B) after ischemia reperfusion. Animals were not treated with MICA. One h ischemia was followed by 1 or 2 h reperfusion. This was further followed by brain mitochondria isolation for gel analysis. DLDH activity was measured by in-gel BN-PAGE assay (upper panel) as described in the text, and DLDH protein content was measured by anti-DLDH Western blot assay (lower panel). Shown are contralateral samples and ipsilateral samples after 1 and 2 h reperfusion. As can be seen in the upper panel, DLDH activity was lost after 1 h reperfusion but recovered after 2 h reperfusion, but protein content did not change.