Fig. 1.

Diagram illustrating the design of the silver CVS container. a A 4.0-cm-tall silver tube, with a 0.5-cm diameter and 0.2-mm thickness of the wall and bottom. b A 2.0-cm-tall PVF cap with a 0.5-cm opening and 0.2-mm thickness of the wall and bottom. c Illustration of the ovarian cortex and an appropriate amount of cryoprotectants in a silver container. The PVF cap was tightly closed on the silver tube with a 0.5-cm overlap by clockwise screwing. The cap was then sealed with paraffin film