Fig. 3.

Confocal three-dimensional reconstructions of microtubules (green) and chromosomes (red) are showing representative patterns used in the classification of spindles as bipolar (A, a′), bipolar with irregularities at either the spindle poles (B, b′) or equator (C, c′). Similarly, chromosomes were classified as aligned (a, a′), mostly aligned with ≤6 chromosomes away from the spindle equatorial region (b, b′) and dispersed with >6 chromosomes away or all chromosomes located throughout the spindle (c, c′). In summary, normal spindle complexes are shown in a′ and b′ and an abnormal spindle complex is shown in c′