Table 1.
Details of the selected genes and primer pairs used for gene expression analysis
| Gene | Primer sequences (5′–3′) | Amplicon size | Reference/Genbank accession no. | Amplification efficiency, % (R 2) |
|---|---|---|---|---|
| GREM1 | F: TGCTGGAGTCCAGCCAAGA; R: GCACCAGTCTCGCTTCAGGTA | 65 | [20] | 97.04 (0.99) |
| HAS2 | F: CCTCATCATCCAAAGCCTG; R: ACATTTCCGCAAATAGTCTG | 138 | [21] | 97.47 (0.99) |
| PTGS2 | F: AGGAGGTCTTTGGTCTGGTG; R: TCTGGAACAACTGCTCATCG | 126 | [21] | 95.02 (0.99) |
| STAR | F: GCCAGGTGTTGAAGGCCA; R: TCTTTAACAGACTTGGAGGCTTCC | 104 | This study (NM_001009243.1) | 93.33 (0.99) |
| SDC2 | F: ATGCGATACACCAACAGCAGGA; R: GAAACCACAACGCTGAGCAAGA | 257 | [22] | 90.35 (0.99) |
| LHR | F: TGACTATGGTTTCTGCTTACCCAA; R: CCATAATGTCTTCACAGGGATTGA | 80 | [23] | 91.41 (0.99) |
| FSHR | F: GCCTGCCCATGGATATTGAC; R: TAGCAACCACAGATGACCACAAA | 97 | [23] | 92.78 (0.99) |
| FGF2 | F: AGTGTGTGCAAACCGTTACCTTGC; R: ATACTGCCCAGTTCGTTTCAGTGC | 172 | [24] | 90.18 (0.99) |
| FGF10 | F: TCCCCACCTGTCCGGCTGCT; R: CGCACATGCCTCCCCGCACT | 183 | This study (NM_001009230.1) | 94.88 (0.99) |
| BCL2 | F: GCCGAGATGTCCAGTCAGC; R: GACGCTCTCCACACACATGAC | 150 | [25] | 94.50 (0.99) |
| BAX | F: CATGGAGCTGCAGAGGATGA; R: GTTGAAGTTGCCGTCGGAAA | 100 | [25] | 98.19 (0.99) |
| IL7RA | F: ACCAAGCTGACACTCCTAC; R: CCATTCACTCCAGAAGCC | 107 | This study (XM_004017053.1) | 96.67 (0.99) |
| HSPA1A | F: CTGATCAAGCGCAACTCCA; R: AGCAGGTTGTTGTCCCGAGT | 131 | [26] | 98.84 (0.99) |
| IFNT | F: CCTGGACCGAATGAACAGA; R: AGGTTGAAGCTCTGCTGGAG | 150 | [26] | 94.55 (0.99) |
| 18S | F: AGAAACGGCTACCACATCCAA; R: CCTGTATTGTTATTTTTCGT | 90 | [27] | 96.09 (0.99) |