Table 3.
Concentration of total, free and ester cholesterol in the blood serum of BALB/c mice treated with probiotic bacteria or probiotic compositions
| Group of mice | Concentration of cholesterol (mg/ml) term of observation after probiotic bacterial strains injection | ||||
|---|---|---|---|---|---|
| Day 4 | Day 9 | Day 15 | Day 21 | Day 30 | |
| Concentration of total cholesterol (sum of free and ester cholesterol), mg/ml | |||||
| Intact mice | 7.64 ± 0.35 | 7.16 ± 0.31 | 7.43 ± 0.17 | 7.83 ± 0.11 | 7.28 ± 0.14 |
| Mice that received FED | 17.71 ± 0.76* | 16.66 ± 0.28* | 15.18 ± 0.37* | 14.09 ± 0.70* | 14.63 ± 0.53* |
| Mice that received FED + L. casei IMV B-7280 | 9.77 ± 0.06* | 8.56 ± 0.15* | 7.37 ± 0.25* | 5.93 ± 0.48*• | 4.56 ± 0.23*• |
| Mice that received FED + L. delbrueckii subsp. bulgaricus ІМV В-7281 | 16.55 ± 0.15* | 14.89 ± 0.48•* | 13.56 ± 0.38•* | 13.81 ± 0.81* | 10.23 ± 0.63•* |
| Mice that received FED + B. animalis VKB | 16.55 ± 0.65* | 14.12 ± 0.36•* | 12.45 ± 0.61•* | 10.35 ± 0.45•* | 9.63 ± 0.63•* |
| Mice that received FED + B. animalis VKL | 15.22 ± 0.38•* | 12.45 ± 0.74•* | 9.64 ± 0.64•* | 8.22 ± 0.39• | 7.15 ± 0.47• |
| Mice that received FED + B. animalis VKB/B. animalis VKL | 14.38 ± 0.21* | 13.22 ± 0.08* | 11.63 ± 0.19* | 9.13 ± 0.59* | 8.91 ± 0.10* |
| Mice that received FED + B. animalis VKL/B. animalis VKB/L. casei IMV B-7280 | 15.88 ± 0.26* | 12.11 ± 0.35•* | 9.03 ± 0.35•* | 7.62 ± 0.95• | 6.11 ± 0.69• |
| Concentration of free cholesterol | |||||
| Intact mice | 4.82 ± 0.36 | 4.34 ± 0.31 | 4.61 ± 0.18 | 5.01 ± 0.11 | 4.46 ± 0.14 |
| Mice that received FED | 12.33 ± 0.77* | 11.27 ± 0.29* | 9.77 ± 0.37* | 8.68 ± 0.71* | 9.21 ± 0.53* |
| Mice that received FED + L. casei IMV B-7280 | 8.41 ± 0.06* | 7.19 ± 0.15* | 5.99 ± 0.25* | 4.53 ± 0.49*• | 3.15 ± 0.23*• |
| Mice that received FED + L. delbrueckii subsp. bulgaricus ІМV В-7281 | 7.12 ± 0.55•* | 6.89 ± 0.62•* | 5.15 ± 0.37• | 5.59 ± 0.84• | 4.85 ± 0.17• |
| Mice that received FED + B. animalis VKB | 11.13 ± 0.82* | 10.67 ± 0.45* | 8.11 ± 0.81* | 8.22 ± 0.37* | 7.78 ± 0.55* |
| Mice that received FED + B. animalis VKL | 10.46 ± 0.71* | 9.79 ± 0.43•* | 8.56 ± 0.33* | 7.16 ± 0.62* | 6.02 ± 0.27•* |
| Mice that received FED + B. animalis VKB/B. animalis VKL | 9.72 ± 0.22* | 8.54 ± 0.08* | 6.93 ± 0.19* | 4.41 ± 0.60* | 4.19 ± 0.10*• |
| Mice that received FED + B. animalis VKL/B. animalis VKB/L. casei IMV B-7280 | 8.65 ± 0.35•* | 8.12 ± 0.41•* | 6.52 ± 0.89•* | 6.03 ± 0.63• | 4.45 ± 0.40• |
| Concentration of ester cholesterol | |||||
| Intact mice | 2.82 ± 0.04 | 2.83 ± 0.03 | 2.82 ± 0.05 | 2.82 ± 0.03 | 2.84 ± 0.01 |
| Mice that received FED | 5.31 ± 0.09* | 5.45 ± 0.14* | 5.44 ± 0.04* | 5.43 ± 0.06* | 5.38 ± 0.08* |
| Mice that received FED + L. casei IMV B-7280 | 1.31 ± 0.02*• | 1.37 ± 0.04*• | 1.42 ± 0.02*• | 1.45 ± 0.02*• | 1.43 ± 0.03*• |
| Mice that received FED + L. delbrueckii subsp. bulgaricus ІМV В-7281 | 9.41 ± 0.05•* | 8.12 ± 0.21•* | 8.37 ± 0.18•* | 8.25 ± 0.16•* | 5.33 ± 0.09* |
| Mice that received FED + B. animalis VKB | 5.47 ± 0.11* | 3.38 ± 0.04•* | 4.24 ± 0.03•* | 2.17 ± 0.03•* | 1.83 ± 0.01•* |
| Mice that received FED + B. animalis VKL | 4.73 ± 0.13*• | 2.65 ± 0.08• | 1.11 ± 0.01•* | 1.05 ± 0.01•* | 1.19 ± 0.02•* |
| Mice that received FED + B. animalis VKB/B. animalis VKL | 4.67 ± 0.11*• | 4.62 ± 0.07*• | 4.75 ± 0.07*• | 4.71 ± 0.07*• | 4.79 ± 0.07*• |
| Mice that received FED + B. animalis VKL/B. animalis VKB/L. casei IMV B-7280 | 7.33 ± 0.04•* | 4.12 ± 0.03•* | 2.55 ± 0.03• | 1.59 ± 0.02•* | 1.69 ± 0.01•* |
| The ratio of esterification (the ratio of ester cholesterol to total cholesterol) | |||||
| Intact mice (control) | 0.35 ± 0.02 | 0.38 ± 0.01 | 0.39 ± 0.02 | 0.37 ± 0.02 | 0.40 ± 0.03 |
| Mice that received FED | 0.28 ± 0.03 | 0.33 ± 0.01 | 0.37 ± 0.01 | 0.38 ± 0.03 | 0.36 ± 0.01 |
| Mice that received FED + L. casei IMV B-7280 | 0.13 ± 0.05 | 0.16 ± 0.02 | 0.19 ± 0.09 | 0.24 ± 0.04 | 0.31 ± 0.03 |
| Mice that received FED + L. delbrueckii subsp. bulgaricus ІМV В-7281 | 0.59 ± 0.04•* | 0.57 ± 0.03•* | 0.64 ± 0.03•* | 0.63 ± 0.04•* | 0.54 ± 0.04•* |
| Mice that received FED + B. animalis VKB | 0.34 ± 0.01 | 0.22 ± 0.02•* | 0.34 ± 0.02 | 0.22 ± 0.01•* | 0.17 ± 0.01•* |
| Mice that received FED + B. animalis VKL | 0.32 ± 0.03 | 0.23 ± 0.02•* | 0.12 ± 0.01•* | 0.14 ± 0.01•* | 0.16 ± 0.01•* |
| Mice that received FED + B. animalis VKB/B. animalis VKL | 0.32 ± 0.09 | 0.34 ± 0.01 | 0.40 ± 0.08 | 0.51 ± 0.02 | 0.54 ± 0.09 |
| Mice that received FED + B. animalis VKL/B. animalis VKB/L. casei IMV B-7280 | 0.47 ± 0.02•* | 0.36 ± 0.01 | 0.27 ± 0.02• | 0.20 ± 0.02•* | 0.29 ± 0.02* |
Significant differences with intact mice are represented by * (P < 0.05); significant difference with obese mice that did not receive probiotic bacteria are presented by • (P < 0.05)