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. 2017 Nov 22;8:1697. doi: 10.1038/s41467-017-01850-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Successful lymphocyte transformation depends on CHK1 expression levels. a Total splenocytes of the indicated genotypes were processed for immunoblotting using the indicated antibodies to assess the impact of MYC on CHK1 protein levels. Each lane represents an independent biological replicate. Two independent experiments are shown (n = 5/genotype in total) b Kaplan–Meier plot analysis showing lymphoma-free survival of mice of the indicated genotypes over an observation period of one year. Eμ-MYC ⁺ n = 31, median survival 106 days, Eμ-MYC ⁺ Chk1 ^+/− n = 41, median survival 205 days. Logrank (Mantel-Cox) p = 0.056 (χ ² = 3.632), Breslow–Gehan–Wilcoxon p = 0.0258 (χ ² = 4.967). c Wild-type C57Bl/6N mice were either left untreated or irradiated with a single dose of 1.75 Gy. Four days after irradiation organs were harvested and processed for western analysis. Each lane represents an independent biological replicate. d Kaplan–Meier analysis showing lymphoma-free survival of WT and Chk1 ^+/− mice exposed to a fractionated irradiation protocol (4× 1.75 Gy) at 4 weeks of age. Median tumor onset: WT (n = 7) 217 days, Chk1 ^+/− (n = 24) 245 days. Logrank (Mantel-Cox) p = 0.0399 (χ ² = 4.221)