Fig. 7.

CHK1 inhibition induces cell cycle arrest in apoptosis-resistant cells. a Murine primary pre B cell lines as described in Fig. 6c, d. Left: western blot analysis ± CHK1 inhibition (500 nM PF-477736). Right: population doublings 48 h after treatment with two different CHK1 inhibitors PF-477736 or CHIR-124 or solvent control (DMSO) (n = 3). b Nalm6 BAX ^−/− BAK ^−/− cells were treated (left) with PF-477736 (1.5 μM) or solvent control (DMSO) and processed for western blot. Right: population doublings as described in a (n = 4). c The BCL2 overexpressing BL-2 cell line was treated with (left) PF-477736 (1.5 μM) or solvent control (DMSO) and processed for western blot. Right: population doublings as described in a from indicated BCL2 overexpressing Burkitt lymphoma cell lines (n = 3). * p53: unspecific band. Bars represent means ± S.E.M., *p < 0.05, **p < 0.01, *** p < 0.001 using unpaired Student´s t test