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. 2017 Nov 23;8:1723. doi: 10.1038/s41467-017-01650-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Comparison of mismatch tolerance of fgRNAs with those of guide RNAs. a Mismatched fgRNAs or gRNAs that differed from human VEGFA target sites by one nucleotide were transfected into HeLa cells. b Comparison of off-target effects between the fgRNAs and conventional gRNAs of LbCpf1. No significant difference was observed between the two types of guide RNAs at four different potential off-target sites. Mismatched nucleotides are colored in red. Error bars indicate s.e.m. (n = 2)