Fig. 2.

Subtelomeric rearrangements in quiescence correspond to duplication of a STE1-extended region. a Top, relative position of the restriction sites in the telomeric and subtelomeric regions within pNSU70 and pNSU64. Both pNSU64 and pNSU70 are representative plasmids that share significant restriction pattern similarity with pNSU65/pNSU21 and pNSU77/pNSU71, respectively²⁹. The position of telomeric (Telo) and subtelomeric probes (STE1 and STE2) used for southern blot are represented. Bottom, genomic DNAs from quiescent cells (ter1+, after 8 days in G0 (+)) and ter1Δ cells (after 1 and 8 days in G0 (1 and 8)) were digested by ApaI, EcoRI, SwaI and NsiI, and southern blotted. The membrane was hybridized with either Telo, STE1, or STE2 probes, as indicated. Ethidium bromide staining DNA was used as a loading control. Asterisks represent the residual signal from STE1 probe. b Schematic representation of an ApaI–ApaI subtelomeric duplication. STE1-Extended region (STEEx) within subtelomeric region of pNSU70 and pNSU64 (pNSU70^×2 and pNSU64^×2). According with this scheme, EcoRI digestions of pNSU64^×2 and pNSU70^×2 generate two DNA fragments of 902 and 1461 bp, and a single DNA fragment of 1533 bp, respectively. These three DNA fragments correspond to the bands that accumulated during quiescence at eroded telomeres. The presence of telomeric repeats at STEEx ends is not established