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. 2017 Nov 22;8:1684. doi: 10.1038/s41467-017-01894-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — STEEx formation in quiescent cells does not depend on heterochromatin assembly machinery. a Analysis of STEEx chromosomes by pulsed-field gel electrophoresis. Gel was stained with ethidium bromide. b–d Genomic DNA from quiescent telomerase positive cells and ter1Δ cells with the indicated genetic backgrounds was digested with EcoRI and southern blotted. Membranes were hybridized with a Telo/STE1 probe. Cells were collected after the indicated days (1, 4, 6, and 8) in quiescence. R shows ter1+ cells collected during replicative growth. STEEx are shown by asterisks