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. 2017 Nov 22;7:16016. doi: 10.1038/s41598-017-16091-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative rate of expression of groES and groEL validate the run-out of mRNA after rifampicin addition in D457 and ALB001 strains. Strains D457 and ALB001 were cultivated in LB medium at 37 °C. At 0.6 OD_600nm rifampicin was added to 200 mg/L to inhibit transcription by RNA polymerase. At different time points, aliquots were taken for RNA extraction and the relative amount of groES and groEL mRNAs was measured by real-time RT-PCR. Values indicate the decrease of the mRNA levels observed as a function of time after rifampicin addition (time 0) and correspond to the average of two independent assays; the standard error is shown.