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Comparison of mRNA half-lives as estimated by Illumina RNA-Seq. 1.9 and RT-qPCR for 4 S. maltophilia D457 genes used for validation of normalization method.
| Gene | Expression (RPKM)a | mRNA Half-life by RNA-Seq (min) | mRNA Half-life by RT-qPCR (min) |
|---|---|---|---|
| rpoD (SMD_3765) | 658 | 2,34 | 2,50 |
| ftsZ (SMD_0648) | 384 | 3,58 | 4,03 |
| gyrA (SMD_2717) | 488 | 2,40 | 2,58 |
| groEL (SMD_3813) | 1879 | 3,49 | 3,67 |
aExpression in mid-exponential phase of S. maltophilia D457 culture growing in LB medium18.
