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. 2017 Nov 22;8:1688. doi: 10.1038/s41467-017-01695-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Demonstration of CRISPR-AID using the reporter yeast strain CT. By transforming the reporter strain with a single plasmid containing an array of 3 gRNAs, transcriptional activation of mCherry a, transcriptional interference of mVenus b, and deletion of an endogenous ADE2 gene c were achieved simultaneously with high efficiency. The inset in c shows a representative result of ADE2 deletion using CRISPR-AID. Error bars represent the mean ± s.d. of biological quadruplicates