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. 2017 Nov 22;7:15989. doi: 10.1038/s41598-017-16262-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genomic rearrangement of the HCMV isolate used in this study. Panel A shows schematic representations of the original Towne strain virus above) and the isolate used in our experiments (below). The unique long (UL) and unique short (US) sequences are bracketed by repeat sequences (a–c), marked by coloured rectangles (brown, pink and green, respectively). In our isolate, the UL/b’ (180887–193945) region of the FJ616285 genome is substituted by the 710–11996 region. To confirm this rearrangement, primers have been designed to in the ends of the UL (P_fw) and b’ (P_rev) regions 243 nt apart. Panel B shows the PCR product of approximately 250 nt. The original gel photo, from which Panel B was cropped is shown in Supplementary Fig. S1.