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. 2017 Nov 22;7:15992. doi: 10.1038/s41598-017-16149-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Metformin (Met) increases the percentage of cells in the G0/G1 phase, and affects the expression of various cell cycle regulatory proteins in HT29, HCT116 and HCT116 p53−/− cells. (a) Flow cytometric analysis of proliferating cells 72 hours after the addition of 5 mM Met. The results are representative of three independent experiments. (b) Immunoblots of p-Rb, cycD1, cycE and c-Myc in cells treated for 72 hours with 5 mM Met or left untreated. β-actin was used as the loading control. Full-size blots are shown in Supplementary Fig. S10. (c) Immunohistochemistry of c-Myc.