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. 2017 Nov 22;7:16021. doi: 10.1038/s41598-017-16310-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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False positive genotyped samples by HRM assay. (A) The difference curves of the false positive for rs4986893 sample (yellow, FP), wild type sample (black, WT), heterozygous sample (green, HET), homozygous mutant sample (red, HOM). (B) Sanger sequencing results for the target SNP allele of rs4986893 HRM genotyping (green in A), and (C), sequencing results for the false positive sample (yellow in A) showing an alternate variant allele. (D) The difference curves of the false positive for rs12248560 sample (yellow, FP), wild type sample (black, WT), heterozygous sample (green, HET). (E) Sanger sequencing results for the target SNP allele of rs12248560 HRM genotyping (green in D), and (F), sequencing results for the false positive sample (yellow in D) showing an alternate variant allele.