Table 2.
Primers sequences for the multiplex HRM PCR amplification. The underlined bases belong to the GC-rich tail.
| Gene | Code | Primers | Length (bp) | Tm (°C) |
|---|---|---|---|---|
| CYP2C19*2 | rs4244285 | CCCACTATCATTGATTATTTCC | 81 | 75.8 |
| CTCCAAAATATCACTTTCCATA | ||||
| CYP2C19*3 | rs4986893 | CCGGCGGCGGAAAACATCAGGATTGTAAGC | 100 | 85.3 |
| CGGCGGCCGCAAAGACTGTAAGTGGTTTCTCA | ||||
| CYP2C19*17 | rs12248560 | AACAAAGTTTTAGCAAACGATTT | 106 | 80 |
| TGAGGTCTTCTGATGCCCAT | ||||
| PON1 | rs662 | TTGTTGCTGTGGGACCTG | 103 | 81.7 |
| TACGACCACGCTAAACCC | ||||
| ABCB1 | rs1045642 | GGGTGGTGTCACAGGAAGA | 107 | 83.9 |
| CCCAGGCTGTTTATTTGAAG |