Figure 4.

Functionalized nanoparticles bearing hard protein corona colocalize with lysosomes in Huh7 cells. Cells were incubated with BSA or RNase (both 50 µM) labelled with Atto633 (red dye) for 1 h. Additionally, cells were incubated with either Si-OH or Si-NH₂ or PS-NH2 NPs (all 50 µg/ml) bearing either BSA or RNase as hard protein corona (both 50 µM). Acidic organelles were stained with LysoTracker probe (Invitrogen, green dye), and the cells were analyzed using confocal microscopy. Colocalization is yellow.