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. 2017 Nov 22;7:16082. doi: 10.1038/s41598-017-16463-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BAT from obese mice showed increased ROS generation with a concomitant increase in antioxidant enzyme activity. Oxidative status in interscapular BAT (left), eWAT (middle) and iWAT (right) from NCD and HFD mice was assessed by determining ROS generation, oxidative damage to lipids, and antioxidant enzymes activity. (A) Total ROS were measured by determining the fluorescent probe DCFH-DA oxidation (DCF RFU: Dichlorofluorescein Relative Fluorescence Units). (B) Combined detection of malondialdehyde and 4-hydroxynonenal as major lipid peroxidation by-products, expressed together as lipoperoxides (LPO). (C–E) Antioxidant activity of the enzymes catalase (CAT) (C), superoxide dismutase (SOD) (D) and glutathione peroxidase (E). Results are represented as mean ± SEM. n = 8–10; *p < 0.05; **p < 0.01.